• Home
  • Contact Us
iBusiness Angel
No Result
View All Result
Saturday, September 23, 2023
  • Home
  • Finance
  • Accounting
  • Sales
  • Business
  • Marketing
  • Home
  • Finance
  • Accounting
  • Sales
  • Business
  • Marketing
No Result
View All Result
No Result
View All Result
Home Business

What You Need to Know About Whole Transcriptome Sequencing

by Martin McMullen
June 20, 2023
in Business
0
What You Need to Know About Whole Transcriptome Sequencing
154
SHARES
1.9k
VIEWS
Share on FacebookShare on Twitter

Whole transcriptome sequencing is next-generation sequencing that generates a complete set of RNA transcripts from a sample. This method is used to study the expression levels and functional roles of genes in an organism or cell type. 

Whole transcriptome sequencing differs from other next-generation sequencing technologies because it produces DNA sequence data and mRNA sequence data. This provides an important resource for studying gene expression patterns and their relationship with genetic variation.

Below are the benefits of whole transcriptome sequencing 

Genome-wide coverage

Whole transcriptome sequencing (WTS) or transcriptomics has been used to study the transcriptomes of a wide range of organisms and tissues. In particular, WTS can be used to study the transcriptomes of cancer cells and has important implications for understanding cancer biology.

The large number of transcripts generated by WTS results in high coverage of the transcriptome, allowing the identification of differences between samples. This is especially useful for identifying novel transcripts or variants in alternative splicing events. The high coverage also allows the detection of rare transcripts, which may not be present in sufficient quantities to be detected by other methods.

Quantitative gene expression analysis

Whole transcriptome sequencing (WTS) is a powerful tool for gene expression analysis. It provides a full picture of the transcriptome, which includes the gene expression levels of all the transcripts in a cell or tissue sample.

WTS has become increasingly popular in recent years due to rapid advances in sequencing technologies. In particular, next-generation sequencing (NGS) technologies have made it possible to perform WTS at an affordable cost, making this technology accessible to more researchers and laboratories than ever before.

Single-cell transcriptomics

WTS is that it can be used to study single cells rather than bulk samples. This allows researchers to compare individual cells with each other and other cell types in the same organism or other organisms.

WTS has been used in several studies to understand how cancer develops and progresses. For example, one study compared healthy and cancerous tissue from breast cancer patients and found that the tumors had different gene expression patterns. This suggests that there may be different stages in the development of breast cancer, which could be important for diagnosis and treatment.

Sorting normal and cancerous cells

By comparing expression patterns between different types of human cells, scientists can determine which genes are associated with specific cancers, normal cellular processes, and other biological features. For example, they can compare immune system T-cells from healthy people with those of people with multiple sclerosis (MS), which involves nerve inflammation throughout the body, resulting in muscle weakness, blurred vision, and other symptoms. Scientists found that T-cells from MS patients had lower levels of certain proteins that help control inflammation than healthy T-cells. This suggests these proteins play an important role in controlling MS symptoms.

Key Takeaway

Whole transcriptome sequencing allows scientists to study a cell’s complete collection of RNA molecules. This method can identify all of the RNA transcripts present in a cell, including those not found on chromosomes. Whole transcriptome sequencing can also be used to study the relative abundance of different types of RNA in cells.

Martin McMullen
Martin McMullen
No Result
View All Result

Archives

  • September 2023
  • August 2023
  • July 2023
  • June 2023
  • May 2023
  • April 2023
  • March 2023
  • February 2023
  • January 2023
  • December 2022
  • November 2022
  • October 2022
  • September 2022
  • August 2022
  • July 2022
  • June 2022
  • May 2022
  • April 2022
  • March 2022
  • February 2022
  • January 2022
  • December 2021
  • November 2021
  • October 2021
  • September 2021
  • August 2021
  • July 2021
  • June 2021
  • May 2021
  • April 2021
  • March 2021
  • February 2021
  • January 2021
  • December 2020
  • November 2020
  • October 2020
  • September 2020
  • August 2020
  • July 2020
  • June 2020
  • May 2020
  • April 2020
  • March 2020
  • February 2020
  • January 2020
  • December 2019
  • November 2019
  • October 2019
  • September 2019
  • August 2019
  • July 2019
  • June 2019
  • May 2019
  • April 2019
  • March 2019

Categories

  • Accounting
  • Auto
  • Business
  • Coworking space
  • Featured
  • Finance
  • Human Resource
  • Industrial Fume Extractors
  • Industry
  • Investment
  • Laboratory
  • Law
  • Legal
  • Manufacturer
  • Marketing
  • Real Estate
  • Sales
  • Tech

Recent Post

Tripple Stabbing on Lonsdale Street

Tripple Stabbing on Lonsdale Street

September 19, 2023
Unlocking the Secrets the Popularity of SkillsFuture Courses in Singapore   

Unlocking the Secrets the Popularity of SkillsFuture Courses in Singapore   

September 16, 2023
Hospital Performance

4 Advantages of Behavioral Health Partnerships for Hospital Performance

September 15, 2023
Pick the Right Attorney

How to Pick the Right Attorney: 5 Tips You Can’t Ignore

September 15, 2023
How Humor Can Be Your Secret Weapon in Sales and Lead Generation

How Humor Can Be Your Secret Weapon in Sales and Lead Generation

September 1, 2023

Advertisement

Recent Post

Tripple Stabbing on Lonsdale Street

Tripple Stabbing on Lonsdale Street

September 19, 2023
Unlocking the Secrets the Popularity of SkillsFuture Courses in Singapore   

Unlocking the Secrets the Popularity of SkillsFuture Courses in Singapore   

September 16, 2023
Hospital Performance

4 Advantages of Behavioral Health Partnerships for Hospital Performance

September 15, 2023

Quick Link

  • Contact Us
  • Finance
  • Accounting
  • Sales
  • Business
  • Marketing

Featured

Copyright © 2023 ibusinessangel.com | All Right Reserved .

No Result
View All Result
  • Home
  • Finance
  • Accounting
  • Sales
  • Business
  • Marketing

Copyright © 2023 ibusinessangel.com | All Right Reserved .

Login to your account below

Forgotten Password?

Fill the forms bellow to register

All fields are required. Log In

Retrieve your password

Please enter your username or email address to reset your password.

Log In